WHAT IS PHOTOPERIODISM | PHYTOCHROMES & VERNALIZATION
What is Photoperiodism? The variations in day length are Galled photoperiod and the phenomenon is called photoperiodism. It is another very important way in which light exerts its influence on living organisms. Let’s discuss in detail photoperiodism in plants are as under;
PHOTOPERIODISM IN PLANTS
In plants photoperiod and temperature affects flowering, fruit and seed reproduction, bud and seed dormancy, leaf fall and germination. Photoperiod affects flowering when shoot meristems start producing floral buds instead of leaves and lateral buds.
Work of Garner and Allard
Effect of photoperiodism was first studied in 1920 by Garner and Allard. They studied that tobacco plant flowers only after exposure to a series of short days. Tobacco plant naturally flowers under same conditions,- in autumn, but flowering could be induced by artificially giving short day treatment in a greenhouse in the summer. With further studies, they were able to classify flowering plants in long-day plants, which require long days for flowering and day-neutral plants flower without being influenced by photoperiod.
Importance of dark period
Later on, further studies indicated that it is really the length of the dark period which is critical. Thus short-day plants are really long-night plants. If they are grown in short days, but the long night is interrupted by a short light period, flowering is prevented. Long-day plants will flower in short days if the long night period is interrupted.
EFFECT OF QUALITY OF LIGHT
Flowering is also influenced by the quality of light. CCocklebura short-day plant, will not flower if its long night is interrupted but experiments revealed that red light was effective in preventing flowering but reversed the effect of red light. It was also demonstrated that the last days’ treatment always determines the response. This response to light intensity and quality led to the discovery of a blue light sensitive Protein pigments, the phytochromes.
Phytochromes exist in two forms P 660 and P 730, P quiescent (inactive) form absorbs red light at a wavelength of 660 is converted to active P 730. P 730 absorbs far-red light of 730 nm and’ converted to P660. In nature, the P660 to P730 conversion takes place daylight and P 730 to P 660 conversion occurs in the dark, Thus dung the day a plant has P 730 phytochrome while during the night it contains more P 660 phytochromes. The presence of either form provides the plants with a means of detecting whether it is in .a light or dart environment. The rate at which P 730 is converted to P 660 provides the plant with a “clock” for measuring the duration of darkness,
Observation and hypothesis: It has been found that red light inhibits flowering in the short day plants but promotes flowering in long day plants under conditions during which flowering normally takes place.
According to hypothesis P 730, converted from P 660 by the absorption of red light, would inhibit flowering in short day plants promote flowering in long day plants. Because P 730 accumulates in the day and diminishes at night, short day plants could flower only if the night were long enough, during which a great amount of P 730 would not be completely inactivated, so that enough P 730 would remain at the end of the night to promote flowering. But now it is generally agreed that the time measuring phenomenon of flowering is not totally controlled by the interconversion of P 660 to P 730.
Other factors: Other factors like presence or absence of light and length of dark or light period also play an important role in flowering.
Florigen: Phytochromes seem to be responsible for the detection of either light or darkness. The biological clock once stimulus causes the production of florigen hormone in leaves, which travel through the phloem to the floral buds, initiating flowering.
Biennial and perennial plants are stimulated to flower by exposure to low temperature. This is called vernalization.
The low-temperature stimulus is received by the shoot apex of a mature stem or embryo of the seed but not by the leaves as in photoperiodism. For some plants, vernalization is an absolute requirement or in some cases, it simply assists in inducing flowering.
Duration of low temperature: The duration of low temperature (Chilling) treatment varies from four days to three months.
Vernalin and Gibberellin: Temperature around 4C is found to be very effective. It stimulates the production of “Vernalin” which induces vernalization. It is now believed that vernalin is not important but actually it is gibberellin.
Importance of Photoperiodism and vernalization
Photoperiodism vernalization serves to synchronize the reproductive behaviour of plants with their environment, ensuring reproduction at favourable times of years. They also ensure that members of the same species flower at the same time, encouraging cross-pollination for genetic variability.